Mining human antibody repertoires
Identifieur interne : 000D16 ( Main/Exploration ); précédent : 000D15; suivant : 000D17Mining human antibody repertoires
Auteurs : Roger R. Beerli [Suisse] ; Christoph Rader [États-Unis]Source :
- mAbs [ 1942-0862 ] ; 2010.
Abstract
Human monoclonal antibodies (mAbs) have become drugs of choice for the management of an increasing number of human diseases. Human antibody repertoires provide a rich source for human mAbs. Here we review the characteristics of natural and non-natural human antibody repertoires and their mining with non-combinatorial and combinatorial strategies. In particular, we discuss the selection of human mAbs from naïve, immune, transgenic and synthetic human antibody repertoires using methods based on hybridoma technology, clonal expansion of peripheral B cells, single-cell PCR, phage display, yeast display and mammalian cell display. Our reliance on different strategies is shifting as we gain experience and refine methods to the efficient generation of human mAbs with superior pharmacokinetic and pharmacodynamic properties.
Url:
DOI: 10.4161/mabs.2.4.12187
PubMed: 20505349
PubMed Central: 3180084
Affiliations:
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Le document en format XML
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Beerli</name><affiliation wicri:level="1"><nlm:aff id="A1">Cytos Biotechnology AG; Schlieren, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Cytos Biotechnology AG; Schlieren</wicri:regionArea></affiliation></author><author><name sortKey="Rader, Christoph" sort="Rader, Christoph" uniqKey="Rader C" first="Christoph" last="Rader">Christoph Rader</name><affiliation wicri:level="2"><nlm:aff id="A2">Experimental Transplantation and Immunology Branch; Center for Cancer Research; National Cancer Institute; National Institutes of Health; Bethesda, MD USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Experimental Transplantation and Immunology Branch; Center for Cancer Research; National Cancer Institute; National Institutes of Health; Bethesda</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">20505349</idno><idno type="pmc">3180084</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180084</idno><idno type="RBID">PMC:3180084</idno><idno type="doi">10.4161/mabs.2.4.12187</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">000749</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000749</idno><idno type="wicri:Area/Pmc/Curation">000749</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000749</idno><idno type="wicri:Area/Pmc/Checkpoint">000593</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000593</idno><idno type="wicri:Area/Ncbi/Merge">000178</idno><idno type="wicri:Area/Ncbi/Curation">000178</idno><idno type="wicri:Area/Ncbi/Checkpoint">000178</idno><idno type="wicri:doubleKey">1942-0862:2010:Beerli R:mining:human:antibody</idno><idno type="wicri:Area/Main/Merge">000D18</idno><idno type="wicri:Area/Main/Curation">000D16</idno><idno type="wicri:Area/Main/Exploration">000D16</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Mining human antibody repertoires</title><author><name sortKey="Beerli, Roger R" sort="Beerli, Roger R" uniqKey="Beerli R" first="Roger R" last="Beerli">Roger R. Beerli</name><affiliation wicri:level="1"><nlm:aff id="A1">Cytos Biotechnology AG; Schlieren, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Cytos Biotechnology AG; Schlieren</wicri:regionArea></affiliation></author><author><name sortKey="Rader, Christoph" sort="Rader, Christoph" uniqKey="Rader C" first="Christoph" last="Rader">Christoph Rader</name><affiliation wicri:level="2"><nlm:aff id="A2">Experimental Transplantation and Immunology Branch; Center for Cancer Research; National Cancer Institute; National Institutes of Health; Bethesda, MD USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Experimental Transplantation and Immunology Branch; Center for Cancer Research; National Cancer Institute; National Institutes of Health; Bethesda</wicri:cityArea></affiliation></author></analytic><series><title level="j">mAbs</title><idno type="ISSN">1942-0862</idno><idno type="eISSN">1942-0870</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Human monoclonal antibodies (mAbs) have become drugs of choice for the management of an increasing number of human diseases. Human antibody repertoires provide a rich source for human mAbs. Here we review the characteristics of natural and non-natural human antibody repertoires and their mining with non-combinatorial and combinatorial strategies. In particular, we discuss the selection of human mAbs from naïve, immune, transgenic and synthetic human antibody repertoires using methods based on hybridoma technology, clonal expansion of peripheral B cells, single-cell PCR, phage display, yeast display and mammalian cell display. Our reliance on different strategies is shifting as we gain experience and refine methods to the efficient generation of human mAbs with superior pharmacokinetic and pharmacodynamic properties.</p></div></front></TEI><affiliations><list><country><li>Suisse</li><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><country name="Suisse"><noRegion><name sortKey="Beerli, Roger R" sort="Beerli, Roger R" uniqKey="Beerli R" first="Roger R" last="Beerli">Roger R. Beerli</name></noRegion></country><country name="États-Unis"><region name="Maryland"><name sortKey="Rader, Christoph" sort="Rader, Christoph" uniqKey="Rader C" first="Christoph" last="Rader">Christoph Rader</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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